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X-GlcU, CHA

Alternate name: BCIG. X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants.

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Product Description

Alternate name: BCIG

X-GlcU (5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, cyclohexylammonium salt) is a chromogenic β-glucuronidase substrate widely used for monitoring E. coli contamination in food (such as meat, dairy products and shellfish, etc) and water, and for the detection of GUS gene expression in plants. X-GlcU also has clinical applications in the assessment of urinary tract infection by detecting the presence of E. coli. The substrate yields a dark blue (λmax 615 nm) precipitate after enzymatic hydrolysis.

  • White solid soluble in water or DMSO
  • Store desiccated at -20°C and protect from light
  • 20H26BrClN2O7
  • MW: 521.8
  • [18656-96-7]

 

References

1. J Food Protect 53(6), 508 (1990).

2. Appl Environ Microbiol 54, 1874 (1988).

3. J Clin Microbiol 27(4), 778 (1989).

4. Plant Cell Reports 13(1), 17 (1993).

5. J Am Soc Horticult Sci 115(4), 686 (1990).

 

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    X-GlcU, Na

    X-GlcU, Na ( 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide, sodium salt), also known as X-GlcA, Na, is similar to X-GlcU, CHA, but has better water solubility.

    Red-β-D-GlcU,CHA

    Red-β-D-GlcU (5-Bromo-6-chloro-3-indolyl-β-D-glucuronide,cyclohexylammmonium salt), also known as Magenta-β-D-GlcA; is similar to X-GlcU, but the product of the substrate is a red (λmax 565 nm) precipitate, which is easier to detect against the background of plant cells.

    Rose-β-D-GlcU,CHA

    Rose-β-D-GlcU (6-Chloro-3-indolyl-β-D-glucuronide,cyclohexylammonium salt), also known as Salmon-β-D-GlcUA, is similar to X-GlcU, but the product of the substrate is a rose-colored (λmax ~ 540 nm) precipitate, which is easier to detect against the background of plant cells.